Multi User of Biochemistry and Instrumental Analysis

The Comprehensive Center for Biochemistry and Instrumental Analysis was established in 2015. Its purpose is to provide specialized technical and infrastructure support for chromatographic analysis (HPLC-UV-FL-IR, CG-FID and CG-MS), to evaluate the activity. antioxidant (ROS and RNS, for example) and carry out bioaccessibility and bioavailability assays of foods and natural products. It serves academic users (professors, postdocs, postgraduates and undergraduate students) from the University of São Paulo and other public or private universities. In addition, it also responds to requests from companies and organizations and private research centers operating in the food or related field.

Steering Committee

Prof. Severino Matias de Alencar - ESALQ / USP (President)

Prof. Carmen Josefina Contreras Castillo - ESALQ / USP (Vice President)

Adna Prado Massarioli - ESALQ / USP (Head of Equipment Holder)

Regina Helena Gonçalves - ESALQ / USP (Substitute Responsible for Equipment)


Users Committee

Profa. Daniele Fernanda Maffei - Full Member of ESALQ

 

Teaching member and respective alternate of USP Units users of Central Multiuser

Prof. Carmen Sílvia Fávaro Trindade - Full Member belonging to FZEA

Prof. Marco Antonio Trindade - Substitute Member of FZEA


Postgraduate student member and respective alternate

Anna Paula de Souza Silva - Full Member (PPGCTA)

Pollyanna Souza Batista - Alternate Member (PPGCTA)


Member of institutions outside the University and, optionally, his alternate

Prof. Masaharu Ikegaki - Federal University of Alfenas (Unifal)
To access and use available resources, the user must be registered with the Analytical Center, at the address: http://uspmulti.prp.usp.br/index.php.

To access and use available resources, the user must be registered with the Analytical Center at the address: http://uspmulti.prp.usp.br/pagina_result_detalhes.php?id=23.

Reservations for use will follow the chronological order of requests.

Equipment / Services:

	High Performance Liquid Chromatograph (HPLC) coupled with Ultraviolet (UV), Fluorescence (FL) and Refractive Index (IR) detectors Services: Analysis of tocopherols (α, β, γ and δ), carotenoids and cholesterol in foods and / or natural products.
	Flame Ionization Detector (FID) Gas Chromatograph Services: Analysis of fatty acids profile in foods of animal and vegetable origin, in fresh or processed form.
	Gas Chromatograph Coupled with Flame Ionization Detector (FID) and On-Column Injection Services: Determination of mono, di and triacylglycerols and free and total glycerol in biodiesel
	Mass Spectrometry Coupled Gas Chromatograph (CG-MS) Services: Analysis of volatile, semi-volatile and non-volatile compounds.
	High Performance Liquid Chromatograph (HPLC) coupled with diode array (DAD) and fluorescence (FL) detectors Services: Determination of phenolic compounds and vitamin C (ascorbic acid and dehydroascorbic acid) in food and natural products.
	Microplate reader with UV / visible reading, fluorescence and luminescence Services: Determination of deactivating capacity reactive oxygen species (peroxyl radical, superoxide radical, hydroxyl radical, hydrogen peroxide and hypochlorous acid) and nitrogen (nitric oxide radical).
	Microplate reader with visible reading system Services: Determination of the content of phenolics and total flavonoids; antioxidant activity in vitro by ABTS, DPPH and FRAP methods.
	Karl Fischer Titrator Service: Determination of moisture content.
	Rancimat Services: Evaluation of oxidative stability of oils and fats.
	Freeze Dryer Services: Freeze drying of solid and liquid samples.
	In vitro bioaccessibility assays Services: Bioaccessibility (in vitro gastrointestinal digestion) assessment of natural foods and products.
	Caco-2 human cell epithelial transport assays Services: Evaluation of epithelial transport in human Caco-2 cells, gastrointestinal digestion fraction of food or raw extract and compounds isolated from natural products.